Direct peptide profiling of brain tissue by MALDI-TOF mass spectrometry

Joachim Schachtner; Christian Wegener; Susanne Neupert; Reinhard Predel

doi:10.1007/978-1-60761-535-4_10

Direct peptide profiling of brain tissue by MALDI-TOF mass spectrometry

Methods Mol Biol. 2010:615:129-35. doi: 10.1007/978-1-60761-535-4_10.

Authors

Joachim Schachtner¹, Christian Wegener, Susanne Neupert, Reinhard Predel

Affiliation

¹ Department of Biology, Animal Physiology, Philipps-University, Marburg, Germany.

PMID: 20013205
DOI: 10.1007/978-1-60761-535-4_10

Abstract

Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to analyze the peptide complement in the nervous system of a variety of invertebrate animals from leech to Aplysia and many arthropod species, especially insects and crustaceans. Here, we describe a protocol for direct peptide profiling of defined areas of the central nervous system of insects. With this method, one can routinely and reliably obtain neuropeptide signatures of selected brain areas from various insects.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aplysia* / anatomy & histology
Aplysia* / chemistry
Brain Chemistry*
Central Nervous System / anatomy & histology
Central Nervous System / chemistry*
Peptides / analysis*
Peptides / genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Peptides