Site-directed delivery of ferritin-encapsulated gold nanoparticles

Nanotechnology. 2010 Jan 29;21(4):045305. doi: 10.1088/0957-4484/21/4/045305. Epub 2009 Dec 16.

Abstract

Newly designed porter proteins, which catch gold nanoparticles and deliver the nanoparticles selectively to a silicon dioxide (SiO(2)) surface under the specific conditions were reported. Recombinant apoferritin subunits, each of which has gold-binding peptide and titanium-binding peptide at the C- and N-terminus, respectively, can efficiently encapsulate a gold nanoparticle. The bio-conjugate, a nanogold and surrounding mutant protein subunits, had a property which can deliver itself to the SiO(2) surface through the interaction. In theory, our genetically manipulated apoferritin subunits can encapsulate gold nanoparticles of various sizes, which is a promising property for applications involving surface plasmon resonance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Drug Delivery Systems / methods*
  • Ferritins / chemistry
  • Ferritins / genetics
  • Ferritins / metabolism*
  • Gold / chemistry
  • Gold / metabolism*
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / ultrastructure
  • Models, Molecular
  • Mutation
  • Peptides / chemistry
  • Peptides / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*
  • Silicon Dioxide / chemistry
  • Surface Plasmon Resonance

Substances

  • Peptides
  • Recombinant Proteins
  • Gold
  • Silicon Dioxide
  • Ferritins