Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

Talanta. 2010 Jan 15;80(3):1164-9. doi: 10.1016/j.talanta.2009.09.002.

Abstract

In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (R(ct)) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biosensing Techniques / methods*
  • Caulimovirus / genetics
  • DNA / analysis*
  • DNA / chemistry
  • DNA / genetics
  • Electric Impedance
  • Electrochemistry
  • Glycine max / genetics
  • Nanotubes, Carbon / chemistry*
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / genetics
  • Organisms, Genetically Modified
  • Polymers / chemistry*
  • Promoter Regions, Genetic / genetics
  • Pyrroles / chemistry*
  • Quartz*
  • Spectrum Analysis
  • Staining and Labeling
  • Time Factors

Substances

  • Nanotubes, Carbon
  • Oligodeoxyribonucleotides
  • Polymers
  • Pyrroles
  • Quartz
  • polypyrrole
  • DNA