This article reviews the latest advances in the development of analytical methodologies by CE and CEC for the analysis of different groups of antibiotics. CZE and MEKC have been the most employed CE modes. Analytical information on experimental conditions, detection mode, and LOD are provided to the readers. In addition, the main applications performed in the pharmaceutical, biological, food, and environmental fields have been reviewed emphasizing the sample preparation requirements. The publications reviewed cover the period of time from June 2007 to May 2009.