Abstract
To achieve more accurate and rapid detection of macrolidelincosamide- streptogramin B resistance genes, erm(A), erm(B), and erm(C), we developed a TaqMan probe-based real-time PCR (Q-PCR) method and compared it with conventional PCR (C-PCR), which is the most widely using erm gene identification method. The detection limit of Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterial cells of Staphylococcus aureus. The utilization of Q-PCR might shorten the time to erm detection from 3-4 h to about 50 min. These data indicated that Q-PCR assay appears to be not only highly sensitive and specific, but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay will permit rapid and accurate identification of erm genes from clinical and other samples.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / analysis
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Bacterial Proteins / genetics*
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Cross Infection / microbiology*
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DNA Primers
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DNA Probes*
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Drug Resistance, Multiple, Bacterial / genetics
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Gram-Positive Bacteria / drug effects
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Gram-Positive Bacteria / genetics*
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Gram-Positive Bacteria / isolation & purification
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Gram-Positive Bacterial Infections / microbiology*
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Humans
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Lincosamides / pharmacology
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Macrolides / pharmacology
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Methyltransferases / analysis
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Methyltransferases / genetics*
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Streptogramin B / pharmacology
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Time Factors
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA Primers
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DNA Probes
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Lincosamides
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Macrolides
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Streptogramin B
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Methyltransferases
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ErmA protein, Bacteria
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rRNA (adenosine-O-2'-)methyltransferase