Abstract
Overexpression of antisense chromosomal cis-encoded noncoding RNAss (ncRNAs) in glutamine synthetase I resulted in a decrease in growth, protein synthesis, and antibiotic production in Streptomyces coelicolor. In addition, we predicted 3,597 cis-encoded ncRNAs and validated 13 of them experimentally, including several ncRNAs that are differentially expressed in bacterial hormone-defective mutants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / biosynthesis*
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Blotting, Western
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Gene Expression Profiling
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Gene Expression Regulation, Bacterial*
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Glutamate-Ammonia Ligase / biosynthesis
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Glutamate-Ammonia Ligase / genetics*
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Models, Molecular
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Nucleic Acid Conformation
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RNA, Antisense / genetics
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RNA, Antisense / metabolism*
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RNA, Bacterial / genetics
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RNA, Bacterial / metabolism*
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RNA, Untranslated / genetics
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RNA, Untranslated / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Streptomyces coelicolor / physiology*
Substances
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Anti-Bacterial Agents
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RNA, Antisense
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RNA, Bacterial
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RNA, Untranslated
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glutamine synthetase I
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Glutamate-Ammonia Ligase