Toxicity and bioaccumulation of TiO2 nanoparticle aggregates in Daphnia magna

Abstract

Little is known about the potential ecotoxicity of engineered nanoparticles (NPs) to aquatic organisms. To carefully address this issue, we conducted a comprehensive toxicity assessment, including modified acute (72h) and chronic (21d) toxicity tests as well as nTiO(2) accumulation analysis using Daphnia magna as a model organism. We found that nTiO(2) exerted minimal toxicity to daphnia within the traditional 48h exposure time, but caused high toxicity when the exposure time was extended to 72h. This demonstrated that exposure duration may be a contributing factor in NP-mediated toxicity. Moreover, upon chronic exposure to nTiO(2) for 21d, daphnia displayed severe growth retardation and mortality, as well as reproductive defects. Interestingly, a significant amount of nTiO(2) was found accumulated in daphnia. However, these daphnia displayed difficulty in eliminating nTiO(2) from their body, presenting increased bioconcentration factor (BCF) values. This high level of bioaccumulation may interfere with food intake and ultimately affect growth and reproduction. In summary, long-term exposure of aquatic organisms to nTiO(2) may alter the growing status of these organisms at both individual and population levels, posing risks to aquatic ecosystems.