Abstract
A dimeric 34-kDa antifungal protein, designated as acafusin, was purified from Acacia confusa seeds using Q-Sepharose, DEAE-cellulose, Mono S and Superdex S75. It demonstrated antifungal activity toward Rhizoctonia solani with an IC(50) of 28 microM. Acafusin inhibited the activity of HIV-1 reverse transcriptase mildly with an IC(50) of 80 microM.
MeSH terms
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Acacia / chemistry*
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Antifungal Agents / chemistry*
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Antifungal Agents / isolation & purification
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Antifungal Agents / pharmacology
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Chitin / chemistry
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Chitin / metabolism
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Chromatography, Liquid
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Electrophoresis, Polyacrylamide Gel
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HIV Reverse Transcriptase / antagonists & inhibitors
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Humans
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Hydrogen-Ion Concentration
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Hyphae / chemistry
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Hyphae / metabolism
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Plant Proteins / chemistry*
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Plant Proteins / isolation & purification
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Plant Proteins / metabolism
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Plant Proteins / pharmacology
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Reverse Transcriptase Inhibitors
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Rhizoctonia / chemistry
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Seeds / chemistry*
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Temperature
Substances
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Antifungal Agents
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Plant Proteins
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Reverse Transcriptase Inhibitors
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Chitin
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reverse transcriptase, Human immunodeficiency virus 1
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HIV Reverse Transcriptase