FPLC purification of mouse monoclonal antibodies from ascitic fluid using blue DEAE and thiophilic sorbents

E Juronen; J Parik; P Toomik

doi:10.1016/0022-1759(91)90255-e

FPLC purification of mouse monoclonal antibodies from ascitic fluid using blue DEAE and thiophilic sorbents

J Immunol Methods. 1991 Jan 24;136(1):103-9. doi: 10.1016/0022-1759(91)90255-e.

Authors

E Juronen¹, J Parik, P Toomik

Affiliation

¹ Institute of General and Molecular Pathology, Tartu University, Estonia, U.S.S.R.

PMID: 1995702
DOI: 10.1016/0022-1759(91)90255-e

Abstract

Two fast methods for the purification of mouse monoclonal antibodies from ascites, fluids using Blue-DEAE and 'thiophilic' adsorbent (T gel) in the FPLC system are described. Blue-DEAE chromatography is useful only for IgG1, IgG2a and IgG2b monoclonal antibody purification. T gel is a satisfactory adsorbent for IgG2b purification. Other IgG subclasses and IgM can also be obtained by simple one-step procedures, but the preparations contain small amount of high molecular weight contaminants.

MeSH terms

Animals
Antibodies, Monoclonal / isolation & purification*
Ascitic Fluid / immunology*
Chromatography, Liquid
Ethanolamines
Gels
Immunoglobulin G / isolation & purification*
Mice

Substances

Antibodies, Monoclonal
Ethanolamines
Gels
Immunoglobulin G
2-diethylaminoethanol