C. Diff Quik Chek complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens

J Clin Microbiol. 2010 Feb;48(2):603-5. doi: 10.1128/JCM.01614-09. Epub 2009 Dec 2.

Abstract

We evaluated a single membrane device assay for simultaneously detecting both Clostridium difficile glutamate dehydrogenase (GDH) and toxin A/B antigens against a standard that combines two PCR assays and cytotoxigenic culture. Results showing dual GDH and toxin A/B antigen positives and negatives can be reported immediately as true positives and negatives, respectively. Specimens with discrepant results for GDH and toxins A/B, which comprised 13.2% of the specimens, need to be retested.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Azure Stains / metabolism*
  • Bacterial Proteins / analysis
  • Bacterial Toxins / analysis
  • Cell Culture Techniques / methods
  • Clostridioides difficile / chemistry
  • Clostridioides difficile / isolation & purification*
  • Enterocolitis, Pseudomembranous / diagnosis*
  • Enterotoxins / analysis
  • Feces / microbiology*
  • Glutamate Dehydrogenase / immunology
  • Humans
  • Immunoenzyme Techniques / methods*
  • Methylene Blue / metabolism*
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Xanthenes / metabolism*

Substances

  • Azure Stains
  • Bacterial Proteins
  • Bacterial Toxins
  • Clostridium difficile lethal toxin B
  • Diff Quik
  • Enterotoxins
  • Xanthenes
  • tcdA protein, Clostridium difficile
  • Glutamate Dehydrogenase
  • Methylene Blue