[Determination of militarine in Rhizoma Bletillae by HPLC]

Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(16):2076-8.
[Article in Chinese]

Abstract

Objective: To develop an HPLC method for determination of militarine in Rhizoma Bletillae.

Method: Analysis was carried out on a Symmetry C18 analytical column, and a C18 guard column eluted with mobile phase consisted of acetonitrile and water (37:63) militarine the detection wavelength was set at 244 nm.

Result: The contents of in 12 samples of Rhizoma Bletillae in market were in the ranges of 1.45%-3.32%.

Conclusion: The method is simple, accurate and reproducible, and is suitable for the quality control of Rhizoma Bletillae.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Drugs, Chinese Herbal / analysis*
  • Orchidaceae / chemistry*
  • Succinates / analysis*

Substances

  • Drugs, Chinese Herbal
  • Succinates
  • militarine