Abstract
This study evaluated the proliferative effects of chishao on RSC96, Schwann cells. A dose-dependent proliferative effect of chishao was obtained by methylthiazol tetrazolium (MTT), proliferating cell nuclear antigen (PCNA) Western blotting, and wound healing assays in Schwann cells administered with chishao (0-500 mg/ml), except at 500 mg/ml concentration. The chishao-treated cells also showed a dose-dependent activated fibroblast growth factor-2 (FGF-2) signaling with increased urokinase plasminogen activator (uPA) and decreased plasminogen activator inhibitor-1 (PAI-1), enhanced proliferative proteins, extracellular signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK)-signaling. Using mitogen-actvated protein kinase (MAPK)-signaling chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of chishao on RSC cells were identified to be ERK- and JNK- signaling dependent. Based on the results, applying appropriate doses of chishao to Schwann cells would be a potential approach for enhancing neuron regeneration.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Blotting, Western
-
Cell Line
-
Cell Movement / drug effects
-
Cell Proliferation / drug effects*
-
Dose-Response Relationship, Drug
-
Drugs, Chinese Herbal / pharmacology*
-
Enzyme Inhibitors / metabolism
-
Extracellular Signal-Regulated MAP Kinases / metabolism
-
Fibroblast Growth Factor 2 / metabolism*
-
Humans
-
JNK Mitogen-Activated Protein Kinases / metabolism
-
Mitogen-Activated Protein Kinases / metabolism*
-
Neurons / drug effects
-
Paeonia*
-
Plant Roots
-
Plasminogen Activator Inhibitor 1 / metabolism
-
Plasminogen Activators / metabolism*
-
Proliferating Cell Nuclear Antigen / metabolism
-
Schwann Cells / drug effects*
-
Schwann Cells / metabolism
-
Signal Transduction / drug effects
-
Urokinase-Type Plasminogen Activator / metabolism
-
Wound Healing / drug effects
Substances
-
Drugs, Chinese Herbal
-
Enzyme Inhibitors
-
Plasminogen Activator Inhibitor 1
-
Proliferating Cell Nuclear Antigen
-
Fibroblast Growth Factor 2
-
Extracellular Signal-Regulated MAP Kinases
-
JNK Mitogen-Activated Protein Kinases
-
Mitogen-Activated Protein Kinases
-
Plasminogen Activators
-
Urokinase-Type Plasminogen Activator