Abstract
Sequencing of the genome of Clostridium botulinum strain Hall A revealed a gene (CBO0515), whose putative amino acid sequence was suggestive of the rare enzyme N(5)-(1-carboxyethyl) ornithine synthase. To test this hypothesis, CBO0515 has been cloned, and the encoded polypeptide was purified and characterized. This unusual gene appears to be confined to proteolytic strains assigned to group 1 of C. botulinum.
Publication types
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Research Support, N.I.H., Intramural
MeSH terms
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Amino Acid Oxidoreductases / genetics*
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Amino Acid Oxidoreductases / metabolism*
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Amino Acid Sequence
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Binding Sites / genetics
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Cloning, Molecular
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Clostridium botulinum / enzymology*
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Clostridium botulinum / genetics*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Genome, Bacterial
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Kinetics
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Metabolic Networks and Pathways
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Molecular Sequence Data
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Ornithine / metabolism*
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Substrate Specificity
Substances
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DNA, Bacterial
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Recombinant Proteins
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Ornithine
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Amino Acid Oxidoreductases
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N(5)-(carboxyethyl)ornithine synthase