Electrochemical selective detection of dopamine on microbial carbohydrate-doped multiwall carbon nanotube-modified electrodes

Biotechnol Lett. 2010 Mar;32(3):413-9. doi: 10.1007/s10529-009-0155-6.

Abstract

Microbial carbohydrate-doped multiwall carbon nanotube (MWNT)-modified electrodes were prepared for the purpose of determining if 4-(2-aminoethyl)benzene-1,2-diol (3,4-dihydroxyphenylalanine; dopamine) exists in the presence of 0.5 mM ascorbic acid, a representative interfering agent in neurotransmitter detection. The microbial carbohydrate dopants were alpha-cyclosophorohexadecaose (alpha-C16) from Xanthomonas oryzae and cyclic-(1 --> 2)-beta-d-glucan (Cys) from Rhizobium meliloti. The cyclic voltammetric responses showed that the highest sensitivity (5.8 x 10(-3) mA cm(-2) microM(-1)) is attained with the Cys-doped MWNT-modified ultra-trace carbon electrode, and that the alpha-C16-doped MWNT-modified glassy carbon electrode displays the best selectivity to dopamine (the approximate peak potential separation is 310 mV).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Buffers
  • Calibration
  • Dopamine / analysis*
  • Electrochemical Techniques / methods*
  • Electrodes
  • Ferricyanides / pharmacology
  • Hydrogen-Ion Concentration / drug effects
  • Nanotubes, Carbon / chemistry*
  • Sinorhizobium meliloti / chemistry*
  • Xanthomonas / chemistry*
  • alpha-Cyclodextrins / chemistry
  • alpha-Cyclodextrins / pharmacology*
  • beta-Glucans / chemistry
  • beta-Glucans / pharmacology*

Substances

  • Buffers
  • Ferricyanides
  • Nanotubes, Carbon
  • alpha-Cyclodextrins
  • alpha-cyclosophorohexadecaose
  • beta-Glucans
  • potassium ferricyanide
  • Dopamine