Enzyme-mediated protein refolding

Jun Okada; Tatsuo Maruyama; Konomi Motomura; Kimiko Kuroki; Katsumi Maenaka; Masafumi Sakono; Masahiro Goto

doi:10.1039/b916225a

Enzyme-mediated protein refolding

Chem Commun (Camb). 2009 Dec 14:(46):7197-9. doi: 10.1039/b916225a. Epub 2009 Oct 16.

Authors

Jun Okada¹, Tatsuo Maruyama, Konomi Motomura, Kimiko Kuroki, Katsumi Maenaka, Masafumi Sakono, Masahiro Goto

Affiliation

¹ Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.

PMID: 19921028
DOI: 10.1039/b916225a

Abstract

We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enzymes, Immobilized / metabolism
Methods
Protein Folding*
Protein Renaturation*
Solutions
Urea / metabolism
Urease / metabolism*

Substances

Enzymes, Immobilized
Solutions
Urea
Urease