Abstract
Fusions to the green fluorescent protein (GFP) are an effective way to monitor protein localization. However, altered codon usage in Candida species has delayed implementation of new variants. Examination of three new GFP variants in Candida albicans showed that one has higher signal intensity and increased resistance to photobleaching.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Candida albicans / cytology
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Candida albicans / metabolism*
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Fluorescent Dyes / metabolism
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism*
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Humans
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Microscopy, Fluorescence / methods
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism*
Substances
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Fluorescent Dyes
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Fungal Proteins
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Recombinant Fusion Proteins
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Green Fluorescent Proteins