Identification of sulfurtransferase enzymes in Azotobacter vinelandii

FEBS Lett. 1991 Jan 28;278(2):151-4. doi: 10.1016/0014-5793(91)80105-c.

Abstract

Rhodanese and 3-mercaptopyruvate sulphurtransferase have been identified in A. vinelandii. Two distinct active fractions of the two sulphur transferases were obtained after FPLC ion-exchange chromatography of material partially purified from crude extracts. Rhodanese has been purified to homogeneity, and it consists of one polypeptide chain of Mr ca 25,000. A partial purification of 3-mercaptopyruvate sulphurtransferase was obtained.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azotobacter / enzymology*
  • Electrophoresis, Polyacrylamide Gel
  • Molecular Weight
  • Nitrogen / metabolism
  • Sulfurtransferases / metabolism*
  • Thiosulfate Sulfurtransferase / metabolism*

Substances

  • Sulfurtransferases
  • Thiosulfate Sulfurtransferase
  • 3-mercaptopyruvate sulphurtransferase
  • Nitrogen