Au(0)/POSS (Octa(aminophenyl) Silsesquioxane) composite was synthesized by chemical reduction of auric acid using glycerol as reducing agent. The morphology and physicochemical properties of Au/POSS nanocomposite were characterized by NMR, FT-IR and TEM spectroscopy. The Au/POSS nanocomposite electrode was used for the oxidation of glucose. The results indicate that the Au/POSS nanocomposite film was highly sensitive and suitable for glucose in 0.5 M NaOH. The presence of ascorbic acid in analyte gave no error in detection of glucose for measuring 5 mM glucose, which is the normal physiological level in blood. Further, Au/POSS nanocomposite showed an enhanced antibacterial activity even higher than standard ciprofloxacin. Hence, Au/POSS nanocomposite acts as a strong antibiocides as well as selective electrodes towards glucose oxidations.