Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptake

Org Biomol Chem. 2009 Dec 7;7(23):4973-80. doi: 10.1039/b912134j. Epub 2009 Sep 24.

Abstract

Four new fluorescent derivatives of cholic acid have been synthesized; they incorporate a dansyl moiety at 3alpha-, 3beta-, 7alpha- or 7beta- positions. These cholic acid analogs are UV photoactive and also exhibit green fluorescence. In addition, they have been demonstrated to be suitable for studying the kinetics of bile acid transport by flow cytometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cholic Acids / chemical synthesis*
  • Cholic Acids / chemistry
  • Cholic Acids / metabolism*
  • Dansyl Compounds / chemical synthesis*
  • Dansyl Compounds / chemistry
  • Dansyl Compounds / metabolism*
  • Flow Cytometry
  • Fluorescence
  • Kinetics
  • Liver / cytology
  • Liver / metabolism*
  • Molecular Conformation
  • Photochemistry
  • Rats
  • Stereoisomerism
  • Ultraviolet Rays

Substances

  • Cholic Acids
  • Dansyl Compounds