A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinase

Xiahong Xu; Zhou Nie; Jinhua Chen; Yingchun Fu; Wang Li; Qinpeng Shen; Shouzhuo Yao

doi:10.1039/b913943e

A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinase

Chem Commun (Camb). 2009 Dec 7:(45):6946-8. doi: 10.1039/b913943e. Epub 2009 Oct 16.

Authors

Xiahong Xu¹, Zhou Nie, Jinhua Chen, Yingchun Fu, Wang Li, Qinpeng Shen, Shouzhuo Yao

Affiliation

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.

PMID: 19904356
DOI: 10.1039/b913943e

Abstract

A novel label-free electrochemical strategy for monitoring the activity and inhibition of protein kinase is developed, based on the linkage between the phosphorylated peptide and DNA functionalized Au nanoparticles (DNA-AuNPs) by Zr(4+) and the chronocoulometric response of [Ru(NH(3))(6)](3+) absorbed on the DNA-AuNPs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
DNA / chemistry*
Electrochemical Techniques
Gold / chemistry
Immobilized Proteins / chemistry
Immobilized Proteins / metabolism
Metal Nanoparticles / chemistry
Phosphorylation
Protein Kinases / chemistry
Protein Kinases / metabolism*
Ruthenium / chemistry
Zirconium / chemistry

Substances

Immobilized Proteins
Gold
Ruthenium
DNA
Zirconium
Protein Kinases