Abstract
Considerable efforts have been taken to identify natural peptides as potential bioactive substances. In this study, novel peptide (SHP-1) derived from seahorse (Hippocampus, Syngnathidae) hydrolysate was explored for its inhibitory effects on collagen release in arthritis with the investigation of its underlying mechanism of action. The efficacy of SHP-1 was determined on cartilage protective effects such as inhibition of collagen and GAG release. SHP-1 was able to suppress not only the expression of collagenases 1 and 3, but also the production of NO via down-regulation of iNOS. However, it presented an irrelevant effect on the level of GAG release in chondrocytic and osteoblastic cells. Inhibition of collagen release by SHP-1 is associated with restraining the phosphorylation of NF-kappaB and p38 kinase cascade. Therefore, it could be suggested that SHP-1 has a potential to be used in arthritis treatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cartilage / chemistry
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Cartilage / metabolism
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Cell Line / drug effects
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Collagen / drug effects
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Collagen / metabolism*
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Collagenases / metabolism*
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Dose-Response Relationship, Drug
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Extracellular Matrix / metabolism
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Humans
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Matrix Metalloproteinase 13 / metabolism*
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Matrix Metalloproteinase Inhibitors
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Matrix Metalloproteinases / metabolism
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Molecular Sequence Data
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NF-kappa B / antagonists & inhibitors
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NF-kappa B / metabolism*
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Nitric Oxide / metabolism*
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Nitric Oxide Synthase Type II / metabolism
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Peptides / genetics
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Peptides / metabolism*
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Peptides / pharmacology
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Signal Transduction / drug effects
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Smegmamorpha*
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p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / metabolism*
Substances
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Matrix Metalloproteinase Inhibitors
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NF-kappa B
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Peptides
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Nitric Oxide
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Collagen
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Nitric Oxide Synthase Type II
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p38 Mitogen-Activated Protein Kinases
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Collagenases
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Matrix Metalloproteinase 13
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Matrix Metalloproteinases
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collagenase 1