Objective: To obtain transgenic Pinellia ternata plants resistant to fungus by transfer Chitinase and beta-1,3-Glucanase gene from Trichoderma harzianum.
Method: Using hygromycin phosphotransferase as the selection marker, the Chitinase gene (ech42), beta-1,3-Glucanase gene (gluc78) and both gene pCAMBIA(ech42 + gluc78) driven by CaMV35S promoter were transferred into P. ternata callus via Agrobacterium-mediated transformation.
Result: PCR results confirmed that the regenerants were identified to be transgenic lines and the RT-PCR results confirmed that foreign genes construction were transfer to mRNA. Two foreign genes were inherited stably to T5 generation according to PCR results of the lines.
Conclusion: The results showed that chitinase gene ech42 and beta-1, 3-glucanase gene gluc78 respectively or together introducing and co-integrating into P. ternata