Determination of the 5'-terminal sequence of subgenomic RNA of hepatitis E virus strains in cultured cells

Arch Virol. 2009;154(12):1945-51. doi: 10.1007/s00705-009-0538-y. Epub 2009 Nov 3.

Abstract

Using RNA preparations extracted from PLC/PRF/5 cells transfected with infectious genotype 3 hepatitis E virus (HEV) cDNA clones or inoculated with a fecal suspension containing a genotype 4 HEV, the 5'-terminal sequence of a 2.2-kb subgenomic RNA of genotype 3 and 4 HEVs was determined. Despite an insertion of T after nucleotide 5116 or an ORF3-null mutation in genotype 4 HEV and/or one of the genotype 3 variants, it was found that the subgenomic RNA of genotype 3 and 4 HEVs initiates exclusively at nucleotide 5122 with the common sequence of 5'-GC, which is identical to that of the prototype genotype 1 HEV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions / genetics*
  • Base Sequence
  • Cell Line
  • Culture Media
  • Culture Media, Conditioned
  • Feces / virology
  • Genome, Viral*
  • Genotype
  • Hepatitis E / virology
  • Hepatitis E virus / genetics*
  • Hepatitis E virus / growth & development*
  • Hepatitis E virus / pathogenicity
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • RNA Caps / genetics
  • RNA, Viral / chemistry
  • RNA, Viral / genetics*
  • Sequence Analysis, DNA*
  • Transfection
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • 5' Untranslated Regions
  • Culture Media
  • Culture Media, Conditioned
  • RNA Caps
  • RNA, Viral
  • Viral Proteins