Abstract
Using RNA preparations extracted from PLC/PRF/5 cells transfected with infectious genotype 3 hepatitis E virus (HEV) cDNA clones or inoculated with a fecal suspension containing a genotype 4 HEV, the 5'-terminal sequence of a 2.2-kb subgenomic RNA of genotype 3 and 4 HEVs was determined. Despite an insertion of T after nucleotide 5116 or an ORF3-null mutation in genotype 4 HEV and/or one of the genotype 3 variants, it was found that the subgenomic RNA of genotype 3 and 4 HEVs initiates exclusively at nucleotide 5122 with the common sequence of 5'-GC, which is identical to that of the prototype genotype 1 HEV.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Untranslated Regions / genetics*
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Base Sequence
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Cell Line
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Culture Media
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Culture Media, Conditioned
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Feces / virology
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Genome, Viral*
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Genotype
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Hepatitis E / virology
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Hepatitis E virus / genetics*
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Hepatitis E virus / growth & development*
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Hepatitis E virus / pathogenicity
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Humans
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Molecular Sequence Data
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Open Reading Frames / genetics
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RNA Caps / genetics
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RNA, Viral / chemistry
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RNA, Viral / genetics*
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Sequence Analysis, DNA*
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Transfection
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Viral Proteins / genetics
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Viral Proteins / metabolism
Substances
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5' Untranslated Regions
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Culture Media
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Culture Media, Conditioned
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RNA Caps
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RNA, Viral
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Viral Proteins