Biotinylated multivalent glycoconjugates for surface coating

Methods Mol Biol. 2010:600:67-78. doi: 10.1007/978-1-60761-454-8_5.

Abstract

Systematic studying of biological processes driven by multipoint high-cooperative carbohydrate recognition requires application of multivalent carbohydrates as tools. In this regard polyacrylamides with various pendant carbohydrate residues and labels are probably the most well advanced class of carbohydrate multimerics. Here we describe a synthetic approach to polyacrylamide-based glycoconjugates with biotin tag, with special emphasis to development of carbohydrate biosensors and arrays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / chemistry
  • Animals
  • Biochemistry / methods
  • Biosensing Techniques
  • Biotin / chemistry*
  • Biotinylation*
  • Carbohydrates / chemistry*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Glycoconjugates / chemistry*
  • Glycoproteins / chemistry
  • Mice
  • Models, Chemical
  • Molecular Biology / methods*
  • Polymers / chemistry
  • Solvents
  • Surface Properties

Substances

  • Acrylic Resins
  • Carbohydrates
  • Glycoconjugates
  • Glycoproteins
  • Polymers
  • Solvents
  • Biotin
  • polyacrylamide