Different bacteria species lipopolysaccharide co-exposure with Pseudomonas exotoxin A on multiple organ injury induction

Immunopharmacol Immunotoxicol. 2009;31(4):616-24. doi: 10.3109/08923970902926402.

Abstract

The present study investigated the effect of different bacterial species lipopolysaccharide plus Pseudomonas exotoxin A (LPS/PEA) on the induction of multiple organ injury (MOI). Rats were injected with various LPS from Salmonella (SAE, SAT), E. coli (EB4, EB5), or P. aeruginosa (PAL) and PEA showed a greater mortality in the SAE/PEA and SAT/PEA groups. Histological alterations, serum enzymes, and cytokines changes were severer in the SAE/PEA group than the EB4/PEA or PAL/PEA group. EB4/PEA and PAL/PEA failed to induce MOI, even at the LPS doses increased up to 2-4- and 4-8-fold, respectively. Rats co-treated with Salmonella lipid A/PEA developed severer MOI than the E. coli lipid A/PEA. The results indicated the critical roles of MOI induction, which were related to LPS derived from appropriate bacterial species.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP Ribose Transferases / toxicity*
  • Animals
  • Bacterial Toxins / toxicity*
  • Dose-Response Relationship, Immunologic
  • Exotoxins / toxicity*
  • Lipopolysaccharides / toxicity*
  • Male
  • Multiple Organ Failure / immunology*
  • Multiple Organ Failure / microbiology*
  • Multiple Organ Failure / mortality
  • Multiple Trauma / immunology*
  • Multiple Trauma / microbiology*
  • Multiple Trauma / mortality
  • Pseudomonas aeruginosa Exotoxin A
  • Rats
  • Rats, Wistar
  • Species Specificity
  • Survival Rate
  • Virulence Factors / toxicity*

Substances

  • Bacterial Toxins
  • Exotoxins
  • Lipopolysaccharides
  • Virulence Factors
  • ADP Ribose Transferases