Objective: To study the chemical constituents in the tubers of Dioscorea bulbifera.
Method: Compounds were isolated and purified with silica gel, ODS and Sephadex LH-20 column chromatography, their structures were determined by using spectroscopic methods including MS and NMR.
Result: Fourteen compounds were isolated and identified as stigmasterol (1), mono-arachidin (2), 1,7-bis-(4-hydroxyphenyl)-1E,4E,6E-heptatrien-3-one (3), behenic acid (4), demethyl batatasin IV (5), 2,3'-di-hydroxy-4',5'-dimethoxybibenzyl (6), diosbulbin B (7), diosbulbin D (8), docosyl ferulate (9), 7-bis-(4-hydroxyphenyl) -4E, 6E-heptadien-3-one (10), 5,3,4-trihydroxy-3,7-dimethoxyflavone (11), tristin(12), protocatechuic acid (13), adenosine (14).
Conclusion: Compounds 24, 6, 9, 10, 12, 14 were isolated from the genus Dioscorea for the first time.