[Isolation and determination of anamorph of Shiraia bambusicola]

Zhongguo Zhong Yao Za Zhi. 2009 Jul;34(13):1640-2.
[Article in Chinese]

Abstract

Objective: To isolate and identify the anamorph of Shiraia bambusicola.

Method: Fungus strains were isolated from mature ascospores and stroma. They were identified by means of morphological identification including colony and microscope characteristic, the molecular identification was done by 5.8S-ITS rDNA.

Result: The strains GZDXIFR-171 and GZDXIFR-181 were isolated and obtained with the separation of different methods, which had the same colony morphology. With the universal primers of the ITS1-5.8S rDNA-ITS2, the 5.8S-ITS rDNA sequence of GZDXIFR-171 and GZDXIFR-181 were obtained by the PCR amplification and sequencing. Compared with the published nucleotide sequence of 5.8S-ITS rDNA in NCBI (National Center for Biotechnology Information), GZDXIFR-171 and GZDXIFR-181 were highly identical with S. bambusicola.

Conclusion: The isolated strains GZDXIFR-171 and GZDXIFR-181 were confirmed to be the true anamorph of S. bambusicola.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascomycota / classification*
  • Ascomycota / genetics
  • Ascomycota / isolation & purification*
  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / genetics
  • Molecular Sequence Data
  • Mycological Typing Techniques
  • Phylogeny
  • RNA, Ribosomal, 5.8S / genetics

Substances

  • DNA, Fungal
  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 5.8S