A novel splice variant of FR4 predominantly expressed in CD4+CD25+ regulatory T cells

Immunol Invest. 2009;38(8):718-29. doi: 10.3109/08820130903171003.

Abstract

Folate receptor 4 (FR4) is recently found as a lymphoid tissue specific protein. In this study, we have identified an alternative splicing variant of the FR4 gene from murine splenocytes, termed FR4v, which is almost identical to FR4 cDNA sequence except with the retained 108 bp intron 3 between exon 3 and 4 of FR4 gene. FR4v mRNA encodes a larger protein than FR4 and is constitutively expressed on CD4(+)CD25(+) regulatory T cell (Treg) membrane via a GPI anchor mechanism. Whether FR4v plays a redundant or unique functional role in Tregs should be investigated further in the future.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CD4 Antigens / biosynthesis
  • Cell Separation
  • Cells, Cultured
  • Cloning, Molecular
  • Female
  • Flow Cytometry
  • Glycosylphosphatidylinositols / metabolism
  • Interleukin-2 Receptor alpha Subunit / biosynthesis
  • Introns / genetics
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Protein Isoforms / genetics
  • Protein Isoforms / immunology
  • Protein Isoforms / metabolism
  • Protein Transport
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / immunology
  • Receptors, Cell Surface / metabolism*
  • Spleen
  • T-Lymphocytes, Regulatory / immunology
  • T-Lymphocytes, Regulatory / metabolism*

Substances

  • CD4 Antigens
  • Glycosylphosphatidylinositols
  • Interleukin-2 Receptor alpha Subunit
  • Protein Isoforms
  • Receptors, Cell Surface
  • folate receptor 4, mouse

Associated data

  • GENBANK/ABY56297
  • GENBANK/EU326437