The effect of repeated applications of Bacillus thuringiensis var israeliensis (Bti) and B. sphaericus (Bs) on different entomologic parameters of malaria transmission was investigated in a village in central Côte d'Ivoire. The study area was monitored for potential mosquito breeding sites over a 7-month period. Microbial larvicides were applied once every 3 wk; first Bti (0.8 mg/liter), followed by Bs (10 mg/liter) 3-4 days later. Adult mosquitoes were collected inside and outside sentinel houses in 4 cross-sectional surveys using human landing catch. Repeated applications of Bti and Bs showed a decline in the biting rate of both Anopheles funestus and Anopheles gambiae. Moreover, the entomologic inoculation rate of An. funestus was significantly reduced (from 328 to 142, P = 0.005), whereas that of An. gambiae remained stable. In conclusion, microbial larvicides might play a role in an integrated approach for malaria control.