The method of making use of the technique of fluorescence spectrum to detect advanced glycation endproducts is discussed in the present pape. The emphasis is on the principle and structure of the fluorescence spectrum detecting system. In the end, the authors made use of the system to detect the excitation spectrum at the wavelengths of 365nm, 370nm, 375nm, 380nm and 385nm, respectively, and the authors found that 375nm is the best excitation wavelength. At the same time, the emission spectrum was also detected on the skin of nondiabetic people and patients with diabetes respectively. The result of the experiment indicates that there is a difference distinctly at about 450nm between them, and has proved the feasibility of the system. The detecting system does not need collecting blood sample, is a noninvasive detection technology, and avoids pain and infection to the patients. The process of detection is very rapid and convenient, and the repetitiveness is well. The patient can benefit from it to forecast and diagnose the state of illness such as diabetes, decrepitude and oxidative stress etc conveniently.