Apply the document influx methods, collect monographes and other medical books of the meridian theory in the period of late Ming and early Qing Dynasties, by analysing the content and characteristics of the meridian theory researchs during this period, summarize the main academic trend of the meridian research in this period. The results indicate that the main characteristics of meridian theory research in the late Ming and early Qing Dynasties are: 1) explain the meridian theory closely combined with clinical; 2) embody the academic trend of "understanding the theory in order to apply"; 3) the researchers include acupuncture doctors, other experts of TCM, some confucianists who also acquainted with medical knowledge; 4) the research content follow the theory system and frame established by internal classic on the whole, and with new explanations in some part of the content; 5) the research perspective develops from acupuncture to human physiology and pathology, treatment and all the other aspects. It shows that the meridian research in the late Ming and early Qing Dynasties has its own unique academic trajectory and direction, and has some new explanations in many aspects based on the classical meridian theory.