Establishing a 3D ex vivo culture system for investigations of bone metabolism and biomaterial interactions

ALTEX. 2007:24 Spec No:56-9.

Abstract

A 3D load-providing culture system is presented, which enables human cancellous bone explants to be studied ex vivo. Bone integrity and activity was demonstrated during 2 to 3 weeks of culture. Viable osteocytes and bone specific markers could be identified. Culture conditions were improved using a serum free medium containing TGF-beta3. Therefore this system gives the opportunity to study bone biology, hormonal effects as well as biomaterial interactions ex vivo prior to animal studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Diseases / metabolism
  • Bone and Bones / cytology
  • Bone and Bones / physiology*
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods
  • Cell Survival
  • Cells, Cultured / cytology
  • Cells, Cultured / metabolism
  • Culture Media
  • Humans
  • Osteoblasts / cytology
  • Osteoclasts / cytology
  • Osteocytes / cytology
  • Osteocytes / drug effects
  • Transforming Growth Factor beta3 / pharmacology
  • Weight-Bearing

Substances

  • Culture Media
  • Transforming Growth Factor beta3