Abstract
Controlled induction of the formation of new microvessels, i.e., therapeutic angiogenesis, may be used one day to treat patients that for example had suffered a myocardial infarction. Experimental models of angiogenesis in the heart in vivo substantially stress the animal. We therefore developed a model of angiogenesis of the heart in vitro, where mouse and rat heart pieces are stimulated under controlled conditions in a three dimensional matrix. Capillary-like sprouts emerging in these cultures represent early to midterm steps of angiogenesis and can be quantified to study potential angiogenic compounds and underlying mechanisms.
MeSH terms
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Angiogenesis Inducing Agents / pharmacology*
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Angiotensin II / pharmacology
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Animals
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Becaplermin
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Capillaries / cytology
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Capillaries / drug effects
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Capillaries / physiology*
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Cell Culture Techniques / methods*
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Coronary Circulation / drug effects
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Coronary Circulation / physiology*
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Fibrin / physiology
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Fibroblast Growth Factor 2 / pharmacology
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Gels
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Heart / physiology*
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Humans
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Mice
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Muscle Tonus / drug effects
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Muscle Tonus / physiology
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Myocardium / cytology
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Myocardium / enzymology
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Neovascularization, Physiologic* / drug effects
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Nitric Oxide / pharmacology
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Nitric Oxide Synthase Type II / deficiency
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Platelet-Derived Growth Factor / pharmacology
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Proto-Oncogene Proteins c-sis
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Rats
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Vasodilation / drug effects
Substances
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Angiogenesis Inducing Agents
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Gels
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Platelet-Derived Growth Factor
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Proto-Oncogene Proteins c-sis
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Fibroblast Growth Factor 2
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Angiotensin II
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Becaplermin
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Nitric Oxide
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Fibrin
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Nitric Oxide Synthase Type II