Abstract
Previous study indicated that p27kip1 gene transfer can obviously inhibit the growth of some carcinoma cells. The purpose of this study was to investigate anticarcinoma mechanism of p27kip1. Gastric carcinoma cell strain SGC-7901 was transfected with recombinant adenovirus vector carrying human p27kip1 gene (Ad-p27kip1). Expression of survivin was detected using the western blot method and activity of telomerase was examined by telomeric repeat amplification protocol (TRAP) PCR-ELISA in gastric carcinoma cells. Our results suggest that upregulated p27kip1 can downregulate survivin expression and inhibit telomerase activity in gastric carcinoma cells.
MeSH terms
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Blotting, Western
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Cell Line, Tumor
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Cell Proliferation
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Cyclin-Dependent Kinase Inhibitor p27
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Down-Regulation
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Enzyme-Linked Immunosorbent Assay
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Gene Expression Regulation, Neoplastic
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Humans
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Inhibitor of Apoptosis Proteins
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Microtubule-Associated Proteins / metabolism*
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Polymerase Chain Reaction
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Stomach Neoplasms / enzymology*
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Stomach Neoplasms / genetics
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Stomach Neoplasms / pathology
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Survivin
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Telomerase / antagonists & inhibitors
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Telomerase / genetics
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Telomerase / metabolism*
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Time Factors
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Transfection
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Up-Regulation
Substances
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BIRC5 protein, human
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CDKN1B protein, human
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Inhibitor of Apoptosis Proteins
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Intracellular Signaling Peptides and Proteins
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Microtubule-Associated Proteins
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Survivin
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Cyclin-Dependent Kinase Inhibitor p27
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Telomerase