[Tongxie Yaofang inhibits the contraction of colonic smooth muscle isolated from rats through a mechanism related to calcium mobilization]

Zhong Xi Yi Jie He Xue Bao. 2009 Oct;7(10):958-62. doi: 10.3736/jcim20091009.
[Article in Chinese]

Abstract

Objective: To study the relationship between the inhibitory effects of Tongxie Yaofang, a compound traditional Chinese herbal medicine, on the contraction of the colonic smooth muscle isolated from rats and calcium mobilization.

Methods: By measuring the tension of the isolated colonic smooth muscle strips, the inhibitory effects of Tongxie Yaofang on the contraction induced by acetylcholine (ACh), KCl and exhausting Ca(2+) of internal calcium store were assessed respectively.

Results: Tongxie Yaofang could concentration-dependently inhibit the contraction of isolated rat colonic smooth muscle strips induced by KCl and exhausting the Ca(2+) of internal calcium store. Tongxie Yaofang could also inhibit the tension of the second contractile phase induced by ACh (P<0.01, vs control), but had no influence on the first contractile phase.

Conclusion: Tongxie Yaofang can inhibit the contraction of isolated rat colonic smooth muscle strips mainly by preventing the influx of extracellular Ca(2+), which may be associated with blocking voltage-dependent channel, store-operated channel and receptor-operated channel, but not by preventing the release of internal Ca(2+) from calcium store.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Colon / drug effects*
  • Colon / physiology
  • Drugs, Chinese Herbal / pharmacology*
  • Male
  • Muscle Contraction / drug effects*
  • Muscle, Smooth
  • Rats
  • Signal Transduction / drug effects

Substances

  • Drugs, Chinese Herbal
  • Calcium