The effect of DNA aptamer configuration on the sensitivity of detection thrombin at surface by acoustic method

Chem Commun (Camb). 2009 Nov 7:(41):6303-5. doi: 10.1039/b910981a. Epub 2009 Sep 8.

Abstract

Using an acoustic method we showed that the aptamer configuration, especially those comprised of dimers with binding exosites sensitive to heparin and fibrinogen at thrombin, substantially increases the aptasensor sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Acoustics*
  • Aptamers, Nucleotide / chemistry*
  • Aptamers, Nucleotide / metabolism
  • Binding Sites
  • Biosensing Techniques / methods*
  • Nucleic Acid Conformation
  • Protein Binding
  • Sensitivity and Specificity
  • Thrombin / analysis*
  • Thrombin / metabolism

Substances

  • Aptamers, Nucleotide
  • Thrombin