Abstract
The production of optically pure d-lactic acid via xylose fermentation was achieved by using a Lactobacillus plantarum NCIMB 8826 strain whose l-lactate dehydrogenase gene was deficient and whose phosphoketolase genes were replaced with a heterologous transketolase gene. After 60 h of fermentation, 41.2 g/liter of d-lactic acid was produced from 50 g/liter of xylose.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Fermentation
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Industrial Microbiology*
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L-Lactate Dehydrogenase / genetics
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L-Lactate Dehydrogenase / metabolism
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Lactic Acid / biosynthesis*
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Lactic Acid / metabolism
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Lactobacillus plantarum / enzymology
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Lactobacillus plantarum / genetics
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Lactobacillus plantarum / metabolism*
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Pentose Phosphate Pathway
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Xylose / metabolism*
Substances
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Lactic Acid
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Xylose
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L-Lactate Dehydrogenase