Proline and glycinebetaine confer cadmium tolerance on tobacco bright yellow-2 cells by increasing ascorbate-glutathione cycle enzyme activities

Mohammad Muzahidul Islam; Md Anamul Hoque; Eiji Okuma; Rayhanur Jannat; Mst Nasrin Akhter Banu; Md Sarwar Jahan; Yoshimasa Nakamura; Yoshiyuki Murata

doi:10.1271/bbb.90305

Proline and glycinebetaine confer cadmium tolerance on tobacco bright yellow-2 cells by increasing ascorbate-glutathione cycle enzyme activities

Biosci Biotechnol Biochem. 2009 Oct;73(10):2320-3. doi: 10.1271/bbb.90305. Epub 2009 Oct 7.

Authors

Mohammad Muzahidul Islam¹, Md Anamul Hoque, Eiji Okuma, Rayhanur Jannat, Mst Nasrin Akhter Banu, Md Sarwar Jahan, Yoshimasa Nakamura, Yoshiyuki Murata

Affiliation

¹ Graduate School of Natural Science and Technology, Okayama University, Japan.

PMID: 19809168
DOI: 10.1271/bbb.90305

Abstract

Cadmium (Cd) stress significantly decreased membrane integrity and impaired the ascorbate (ASC)-glutathione (GSH) cycle in tobacco Bright Yellow-2 cells. Exogenous application of proline and glycinebetaine (betaine) significantly restored the membrane integrity and increased the activities of ASC-GSH cycle enzymes under Cd stress without maintenance of the rich ASC or GSH pools. Moreover, proline offered more efficient protection against Cd stress than betaine.

MeSH terms

Ascorbic Acid / metabolism*
Betaine / pharmacology*
Cadmium / toxicity*
Cell Line
Cell Membrane / drug effects
Cell Membrane / metabolism
Glutathione / metabolism*
Glutathione Transferase / metabolism
Hydrogen Peroxide / metabolism
Intracellular Space / drug effects
Intracellular Space / metabolism
Nicotiana / cytology
Nicotiana / drug effects*
Nicotiana / enzymology*
Nicotiana / metabolism
Proline / pharmacology*
Stress, Physiological / drug effects

Substances

Cadmium
Betaine
Proline
Hydrogen Peroxide
Glutathione Transferase
Glutathione
Ascorbic Acid