Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level

Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17735-40. doi: 10.1073/pnas.0907367106. Epub 2009 Oct 1.

Abstract

The use of plasmon coupling in metal nanoparticles has shown great potential for the optical characterization of many biological processes. Recently, we have demonstrated the use of "plasmon rulers" to observe conformational changes of single biomolecules in vitro. Plasmon rulers provide robust signals without photobleaching or blinking. Here, we show the first application of plasmon rulers to in vivo studies to observe very long trajectories of single biomolecules in live cells. We present a unique type of plasmon ruler comprised of peptide-linked gold nanoparticle satellites around a core particle, which was used as a probe to optically follow cell-signaling pathways in vivo at the single-molecule level. These "crown nanoparticle plasmon rulers" allowed us to continuously monitor trajectories of caspase-3 activity in live cells for over 2 h, providing sufficient time to observe early-stage caspase-3 activation, which was not possible by conventional ensemble analyses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Apoptosis / physiology
  • Caspase 3 / chemistry*
  • Caspase 3 / metabolism*
  • Cell Line
  • Enzyme Activation
  • Gold
  • Humans
  • Kinetics
  • Light
  • Metal Nanoparticles / chemistry*
  • Molecular Probe Techniques*
  • Molecular Probes / chemistry*
  • Protein Conformation
  • Scattering, Radiation
  • Signal Transduction
  • Surface Plasmon Resonance

Substances

  • Molecular Probes
  • Gold
  • CASP3 protein, human
  • Caspase 3