Abstract
Identification of proteins in the mammalian growth cone has the potential to advance our understanding of this critical regulator of neuronal growth and formation of neural circuit; however, to date, only one growth cone marker protein, GAP-43, has been reported. Here, we successfully used a proteomic approach to identify 945 proteins present in developing rat forebrain growth cones, including highly abundant, membrane-associated and actin-associated proteins. Almost 100 of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 17 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus their identification presents a significant step forward, providing marker proteins and candidate neuronal growth-associated proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / analysis
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Adaptor Proteins, Signal Transducing / genetics
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Animals
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Animals, Newborn
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Biomarkers / analysis*
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Catenins / analysis
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Catenins / genetics
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Cells, Cultured
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Chromatography, Liquid
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Growth Cones / metabolism*
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Immunoblotting
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Immunohistochemistry / methods
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Mass Spectrometry
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Microscopy, Fluorescence
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Microtubule-Associated Proteins / analysis
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Microtubule-Associated Proteins / genetics
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Nerve Growth Factors / analysis
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Nerve Growth Factors / genetics
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / genetics
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Neurons / cytology
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Neurons / metabolism
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Prosencephalon / embryology
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Prosencephalon / growth & development
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Prosencephalon / metabolism
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Proteome / analysis*
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Proteome / genetics
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Proteomics / methods*
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RNA Interference
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Rats
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Rats, Transgenic
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Time Factors
Substances
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Adaptor Proteins, Signal Transducing
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Biomarkers
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Catenins
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Dpysl5 protein, rat
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Microtubule-Associated Proteins
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Nerve Growth Factors
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Nerve Tissue Proteins
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Proteome
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microtubule-associated protein 1B
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Green Fluorescent Proteins