Characterization of a dammarenediol synthase in Centella asiatica (L.) Urban

Plant Physiol Biochem. 2009 Nov-Dec;47(11-12):998-1002. doi: 10.1016/j.plaphy.2009.08.001. Epub 2009 Aug 13.

Abstract

To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative beta-amyrin synthase [Plant Cell Rep, 24:304-311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C. asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkyl and Aryl Transferases / genetics*
  • Amino Acid Sequence
  • Centella / enzymology*
  • Centella / genetics
  • Galactose
  • Gene Expression*
  • Genes, Plant*
  • Intramolecular Transferases
  • Mutation
  • Phylogeny
  • Plant Proteins / genetics*
  • Saccharomyces cerevisiae / genetics
  • Saponins / biosynthesis*
  • Saponins / genetics
  • Sequence Homology
  • Triterpenes

Substances

  • Plant Proteins
  • Saponins
  • Triterpenes
  • dammarenediol
  • Alkyl and Aryl Transferases
  • Intramolecular Transferases
  • lanosterol synthase
  • Galactose