Screening of a large global Aspergillus fumigatus species complex collection by using a species-specific microsphere-based Luminex assay

J Clin Microbiol. 2009 Dec;47(12):4171-2. doi: 10.1128/JCM.01415-09. Epub 2009 Sep 30.

Abstract

A microsphere-based Luminex assay was developed and validated for rapid identification of Aspergillus fumigatus from the other species within the A. fumigatus species complex (section Fumigati). This molecular tool was then employed to screen 499 clinical A. fumigatus species complex isolates collected from multiple medical centers throughout the world with results demonstrating the exclusive presence of A. fumigatus.

Publication types

  • Evaluation Study

MeSH terms

  • Aspergillosis / diagnosis*
  • Aspergillosis / microbiology
  • Aspergillus fumigatus / classification*
  • Aspergillus fumigatus / genetics*
  • Aspergillus fumigatus / isolation & purification
  • DNA Probes / genetics
  • DNA, Fungal / analysis
  • DNA, Fungal / genetics
  • Humans
  • Microspheres*
  • Molecular Diagnostic Techniques / methods*
  • Sensitivity and Specificity
  • Species Specificity

Substances

  • DNA Probes
  • DNA, Fungal