Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat

J G Lewis; J L Weber; M B Petersen; S A Slaugenhaupt; A Kwitek; P E May; A C Warren; A Chakravarti; S E Antonarakis

doi:10.1016/0888-7543(90)90300-j

Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat

Genomics. 1990 Oct;8(2):400-2. doi: 10.1016/0888-7543(90)90300-j.

Authors

J G Lewis¹, J L Weber, M B Petersen, S A Slaugenhaupt, A Kwitek, P E May, A C Warren, A Chakravarti, S E Antonarakis

Affiliation

¹ Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

PMID: 1979059
DOI: 10.1016/0888-7543(90)90300-j

Abstract

A (GT)n repeat within the anonymous DNA sequence D21S156 was shown to be highly polymorphic in DNA from members of the 40 CEPH families. At least 12 alleles of this locus were recognized by electrophoresis on polyacrylamide gels of DNA amplified by the polymerase chain reaction (PCR) using primers flanking the (GT)n repeat. The polymorphism information content was 0.82. PCR amplification of DNA from somatic cell hybrid lines mapped D21S156 to human chromosome 21 and linkage analysis localized this marker close to the loci ETS2, D21S3, and HMG14 on chromosomal band 21q22.3. This polymorphism is highly informative and can serve as an anchor locus for human chromosome 21.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Alleles
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 21*
Genetic Markers*
Humans
Lod Score
Molecular Sequence Data
Pedigree
Polymorphism, Restriction Fragment Length*

Substances

Genetic Markers

Grants and funding

etc