The design, fabrication and characterization of a miniaturized, mechanically-actuated 12-channel peristaltic pump for microfluidic applications and built from simple, low-cost materials and fabrication methods is presented. Two pump configurations are tested, including one which reduces pulsating flow. Both use a monolithic PDMS pumping inlay featuring three-dimensional geometries favourable to pumping applications and 12 wholly integrated circular channels. Flow rates in the sub-microL min(-1) to microL min(-1) range were obtained. Channel-to-channel flow rate variability was comparable to a commercial pumping system at lower flow rates. The small footprint, 40 mm by 80 mm, of the micropump renders it portable, and allows its use on microscope stages adjacent to microfluidic devices, thus reducing system dead volumes. The micropump's design allows potential use in remote and resource-limited locations.