Abstract
P68 RNA helicase is a prototypical DEAD box RNA helicase. The protein plays a very important role in early organ development and maturation. Consistent with the function of the protein in transcriptional regulation and pre-mRNA splicing, p68 was found to predominately localize in the cell nucleus. However, recent experiments demonstrate a transient cytoplasmic localization of the protein. We report here that p68 shuttles between the nucleus and the cytoplasm. The nucleocytoplasmic shuttling of p68 is mediated by two nuclear localization signal and two nuclear exporting signal sequence elements. Our experiments reveal that p68 shuttles via a classical RanGTPase-dependent pathway.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / genetics
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Animals
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Cell Nucleus / enzymology*
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Cell Nucleus / genetics*
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Cytoplasm / enzymology*
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Cytoplasm / genetics*
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DEAD-box RNA Helicases / genetics*
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DEAD-box RNA Helicases / metabolism*
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Mice
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NIH 3T3 Cells
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Nuclear Export Signals / genetics
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Nuclear Localization Signals / genetics
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Nuclear Localization Signals / metabolism
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Protein Transport / genetics
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Signal Transduction / genetics
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ran GTP-Binding Protein / genetics
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ran GTP-Binding Protein / metabolism
Substances
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Nuclear Export Signals
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Nuclear Localization Signals
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Ran protein, mouse
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DEAD-box RNA Helicases
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ran GTP-Binding Protein