DNA vaccine encoding avian influenza virus H5 and Esat-6 of Mycobacterium tuberculosis improved antibody responses against AIV in chickens

Sara Oveissi; Abdul Rahman Omar; Khatijah Yusoff; Fatemeh Jahanshiri; Sharifah Syed Hassan

doi:10.1016/j.cimid.2009.08.004

DNA vaccine encoding avian influenza virus H5 and Esat-6 of Mycobacterium tuberculosis improved antibody responses against AIV in chickens

Comp Immunol Microbiol Infect Dis. 2010 Dec;33(6):491-503. doi: 10.1016/j.cimid.2009.08.004. Epub 2009 Sep 24.

Authors

Sara Oveissi¹, Abdul Rahman Omar, Khatijah Yusoff, Fatemeh Jahanshiri, Sharifah Syed Hassan

Affiliation

¹ Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

PMID: 19781778
DOI: 10.1016/j.cimid.2009.08.004

Abstract

The H5 gene of avian influenza virus (AIV) strain A/chicken/Malaysia/5744/2004(H5N1) was cloned into pcDNA3.1 vector, and Esat-6 gene of Mycobacterium tuberculosis was fused into downstream of the H5 gene as a genetic adjuvant for DNA vaccine candidates. The antibody level against AIV was measured using enzyme-linked immunosorbent assay (ELISA) and haemagglutination inhibition (HI) test. Sera obtained from specific-pathogen-free chickens immunized with pcDNA3.1/H5 and pcDNA3.1/H5/Esat-6 demonstrated antibody responses as early as 2 weeks after the first immunization. Furthermore, the overall HI antibody titer in chickens immunized with pcDNA3.1/H5/Esat-6 was higher compared to the chickens immunized with pcDNA3.1/H5 (p<0.05). The results suggested that Esat-6 gene of M. tuberculosis is a potential genetic adjuvant for the development of effective H5 DNA vaccine in chickens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic
Animals
Antibodies, Viral / blood
Antibodies, Viral / immunology
Antigens, Bacterial / genetics*
Antigens, Bacterial / immunology
Bacterial Proteins / genetics*
Bacterial Proteins / immunology
Blotting, Western
Chickens / immunology*
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique, Indirect
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus / genetics*
Hemagglutinin Glycoproteins, Influenza Virus / immunology
Influenza A Virus, H5N1 Subtype / genetics
Influenza A Virus, H5N1 Subtype / immunology*
Influenza Vaccines / immunology*
Influenza in Birds / immunology*
Influenza in Birds / prevention & control
Reverse Transcriptase Polymerase Chain Reaction
Vaccines, DNA / immunology*
Vaccines, DNA / virology

Substances

Adjuvants, Immunologic
Antibodies, Viral
Antigens, Bacterial
Bacterial Proteins
ESAT-6 protein, Mycobacterium tuberculosis
Hemagglutinin Glycoproteins, Influenza Virus
Influenza Vaccines
Vaccines, DNA
hemagglutinin, avian influenza A virus