Objective: To assess the feasibility of using MTT assay for detecting the apoptosis of osteosarcoma cells following chemotherapy.
Methods: The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope.
Results: MTT staining accurately identified apoptosis of the osteosarcoma cells, and the apoptotic cells were easily distinguished from normal cells and dead cells.
Conclusion: MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.