Objective: To study the enrichment and purification of total saponins from the leaves of A. trifoliatus.
Methods: Using the total saponins from the leaves of A. trifoliantus as the standard index, we examinad the capability of static adsorption and desorption to select the optimum macroporous adsportion resin. Then we studied the purification technological parameters such as pH value, the dynamic adorption speed of flow, the best type quantity, the eluent density and so on.
Results: The optimum purification conditions were as follows: the type of macroporous adsorption resin was AB-8, the pH value of absorbing solution was 5-6, the absorbing velocity of flow was 1.0 mL/min, the concentration of ethanol as elutent was 70%, the proportion of the amount of absorbing solution and resins was 4:1, the proportion of the amount of elutent and resins was 4:1, the eluting velocity of flow was 2.0 mL/min.