Ethylene and cold participate in the regulation of LeCBF1 gene expression in postharvest tomato fruits

FEBS Lett. 2009 Oct 20;583(20):3329-34. doi: 10.1016/j.febslet.2009.09.029. Epub 2009 Sep 18.

Abstract

C-repeat/dehydration-responsive element binding factor (CBF) is a transcription factor regulating cold response in plants, of which little is known in fruits. We showed a double-peak expression pattern of Lycopersicon esculentum putative transcriptional activator CBF1 (LeCBF1) in mature green fruit. The peaks appeared at 2 and 16 h after subjection to cold storage (2 degrees C). The second peak was coincident with, and thus caused by a peak in endogenous ethylene production. We showed that LeCBF1 expression was regulated by exogenous ethylene and 1-methylcyclopropene, and was not expressed without cold induction. LeCBF1 expression was different in the five maturation stages of fruits, but expression peaked at 2 h at all stages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cold Temperature*
  • Cyclopropanes / metabolism
  • Ethylenes / metabolism*
  • Fruit* / genetics
  • Fruit* / metabolism
  • Gene Expression Regulation, Plant*
  • Plant Proteins* / genetics
  • Plant Proteins* / metabolism
  • Solanum lycopersicum* / genetics
  • Solanum lycopersicum* / metabolism
  • Transcription Factors* / genetics
  • Transcription Factors* / metabolism

Substances

  • Cyclopropanes
  • Ethylenes
  • Plant Proteins
  • Transcription Factors
  • ethylene
  • 1-methylcyclopropene