Electroporation based on hydrodynamic focusing of microfluidics with low dc voltage

Biomed Microdevices. 2010 Feb;12(1):35-40. doi: 10.1007/s10544-009-9355-z.

Abstract

Microfluidics-based cell electroporation has many advantages in delivering small molecules into cells. In this study, hydrodynamic focusing of fluids with different conductivities has been used for high through-put cell electroporation at low voltage (<3 V) of continuous direct current (dc) power. Simulation results showed that an input voltage of only 1.5 V could generate an electric field intensity of about 1.17 kV cm(-1) across the cell suspension flow in the squeezed area. The electropermeation of yeast cell was observed, showing a permeabilization percentage up to 70%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Membrane Permeability / physiology*
  • Cell Membrane Permeability / radiation effects*
  • Computer Simulation
  • Computer-Aided Design
  • Electromagnetic Fields
  • Electroporation / instrumentation*
  • Electroporation / methods*
  • Equipment Design
  • Equipment Failure Analysis
  • Humans
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods*
  • Models, Biological*